BLOCK-iT™ RNAi Designer
The easiest way to design effective RNAi molecules for great results   See also: Synthetics for in vivo RNAi      Target Design Options:  Stealth RNAi™ siRNA miR RNAi shRNA siRNA to Stealth RNAi™ siRNA siRNA to shRNA
Step 1: Enter an accession number or provide a nucleotide sequence   Accession number: OR Nucleotide sequence: Enter only A, C, G, T, and U. See the online Help for additional information Step 2: If you entered an accession number in Step 1, select regions for target design   Open reading frame (ORF) 5' UTR 3' UTR Step 3: Choose database for Blast Human - Homo sapiens Mouse - Mus musculus Rat - Rattus norvegicus Cattle - Bos taurus Pig - Sus scrofa Dog - Canis familiaris Frog - Xenopus laevis Chicken - Gallus gallus Fruitfly - Drosophila melanogaster Worm - Caenorhabditis elegans Zebrafish - Danio rerio Mosquito - Anopheles gambiae No blast   NOTE: BLAST is used to compare input sequence with sequences in the database to find unique regions against which to design RNAi targets. The databases contain representative gene sequences for that species. Blast databases were updated on March 23, 2013 and the design output reflects the most up-to-date designs. Step 4: Choose minimum and maximum G/C percentage   Minimum G/C percentage: 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90% Maximum G/C percentage: 20% 25% 30% 35% 40% 45% 50% 55% 60% 65% 70% 75% 80% 85% 90%   Step 5: Click "RNAi Design" to design your miR RNAi molecules (for cloning into Invitrogen's pcDNA™6.2-GW/miR and pcDNA™6.2-GW/EmGFP-miR vectors)   Guarantee: The BLOCK-iT™ RNAi Designer uses an optimized, proprietary algorithm to design miRNA sequences with 100% homology to their target, so that upon processing their activity will result in cleavage of that target. The BLOCK-iT™ RNAi Designer is such an effective tool for the design of miRNAs that if you order oligos corresponding to two miRNAs designed by the BLOCK-iT™ RNAi Designer, we guarantee that at least one will give greater than 70% knockdown of target RNA, given that transfection efficiency in your experiment is at least 80%.