BLOCK-iT™ RNAi Designer
The easiest way to design effective RNAi molecules for great results
See also:
Synthetics for in vivo RNAi
Target Design Options:
Stealth RNAi™ siRNA
miR RNAi
shRNA
siRNA to Stealth RNAi™ siRNA
siRNA to shRNA
Convert siRNA to shRNA
To design shRNA based on existing siRNA for cloning into Invitrogen's vectors, enter the sense siRNA sequence, select one of the default loop sequences or enter a custom loop sequence, select appropriate vector and click 'Design shRNA Oligo'. Select
pENTR™/H1/TO vector
for inducible expression and
pENTR™/U6
for constitutive expression.
Sense siRNA Sequence
Default Loop Sequence
Custom Loop Sequence
Vector
CGAA
AACG
GAGA
pENTR™/H1/T0
pENTR™/U6